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Cell Signaling Technology Inc
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Image Search Results
Journal: Arthritis Research & Therapy
Article Title: p16 INK4a and its regulator miR-24 link senescence and chondrocyte terminal differentiation-associated matrix remodeling in osteoarthritis
doi: 10.1186/ar4494
Figure Lengend Snippet: MiR-24 repression correlates with p16 INK4a induction in interleukin-1-beta (IL-1β)-treated chondrocytes and osteoarthritis (OA) cartilage. OA human primary chondrocytes are placed in pellet and treated with IL-1β at 10 ng/mL for 5 days. (A) List of microRNAs downregulated by IL-1β. (B,C) Gene expression analysis was performed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for miR-24 and p16 INK4a (n = 3). (D) Luciferase/β-gal activities of promoter miR-24-2 cluster after IL-1β treatment (n = 3). Data are shown as mean ± standard deviation (SD). MiR-24 repression in OA cartilage samples is shown. (E,F) MiR-24 repression in OA cartilage: gene expression for miR-24 and p16 INK4a on OA (n = 5) and healthy (n = 6) cartilage samples. * P <0.05, ** P <0.01, *** P <0.001.
Article Snippet: Cells were then lysed according to the dual
Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Luciferase, Standard Deviation
Journal: Stem Cell Research & Therapy
Article Title: Human platelet lysate produced from leukoreduction filter contents enables sufficient MSC growth
doi: 10.1186/s13287-025-04329-y
Figure Lengend Snippet: Cell senescence analysis between different supplements. ( A ) β-gal staining of the MSC treated with either f-hPL or FBS after P12. Significantly high signals are seen in the FBS group. ( B ) Fluorescence intensity of MSC treated with different supplements were compared regarding different passages. human AB serum exhibited a higher trend of β-gal expression as early as P8, whereas FBS and f-hPL showed lower levels of expression. MSCs treated with FBS exhibited substantial β-gal expression at P12, whereas f-hPL-treated MSCs displayed comparatively lower expression. n = 3 each, Bar = 100 μm
Article Snippet: Cells were stained using the
Techniques: Staining, Fluorescence, Expressing